Leptolyngbya sp. XZMQ and Bacillus XZM co-inoculation reduced sunflower arsenic toxicity by regulating rhizosphere microbial structure and enzyme activity.

Microorganisms are of great significance for arsenic (As) toxicity amelioration in plants as soil fertility is directly affected by microbes. In this study, we innovatively explored the effects of indigenous cyanobacteria (Leptolyngbya sp. XZMQ) and plant growth-promoting bacteria (PGPB) (Bacillus XZM) on the growth and As absorption of sunflower plants from As-contaminated soil. Results showed that single inoculation and co-inoculation stimulated the growth of sunflower plants (Helianthus annuus L.), enhanced enzyme activities, and reduced As contents. In comparison to the control group, single innoculation of microalgae and bacteria in the rhizosphere increased extracellular polymeric substances (EPS) by 21.99% and 14.36%, respectively, whereas co-inoculation increased them by 35%. Compared with the non-inoculated group, As concentration in the roots, stems and leaves of sunflower plants decreased by 38%, 70% and 41%, respectively, under co-inoculation conditions. Inoculation of Leptolyngbya sp. XZMQ significantly increased the abundance of nifH in soil, while co-inoculation of cyanobacteria and Bacillus XZM significantly increased the abundance of cbbL, indicating that the coupling of Leptolyngbya sp. XZMQ and Bacillus XZM could stimulate the activity of nitrogen-fixing and carbon-fixing microorganisms and increased soil fertility. Moreover, this co-inoculation increased the enzyme activities (catalase, sucrase, urease) in the rhizosphere soil of sunflower and reduced the toxic effect of As on plant. Among these, the activities of catalase, peroxidase, and superoxide dismutase decreased. Meanwhile, co-inoculation enables cyanobacteria and bacteria to attach and entangle in the root area of the plant and develop as symbiotic association, which reduced As toxicity. Co-inoculation increased the abundance of aioA, arrA, arsC, and arsM genes in soil, especially the abundance of microorganisms with aioA and arsM, which reduced the mobility and bioavailability of As in soil, hence, reduced the absorption of As by plants. This study provides a theoretical basis for soil microbial remediation in mining areas.

Indigenous cyanobacteria enhances remediation of arsenic-contaminated soils by regulating physicochemical properties, microbial community structure and function in soil microenvironment.

Biocrust was widely used for the immobilization and removal of arsenic (As) in drainage systems of rice fields and mining areas. In this study, the role of an indigenous cyanobacteria (Leptolyngbya sp. XZMQ) was explored in the bioremediation of As-contaminated farmland and tailing soil. After 80 d of inoculation with cyanobacteria, total As (As(T)) accumulated in the cyanobacterial crust of farmland and tailing soil was 279.89 mg kg-1 and 269.57 mg kg-1, respectively, and non-EDTA exchangeable fraction was the major fraction of it. The As(T) in farmland and tailing soil of micro-environment decreased by 10.76% and 12.73%, respectively. Meanwhile, the available As (As(a)) decreased by 21.25% and 27.65%, respectively. The XRD results showed that hematite and SiO2 existed in cyanobacterial crust of farmland and tailing soil. FTIR spectra indicated that the adsorption of As in cyanobacterial crust was mediated by OH and CO. After inoculation of Leptolyngbya sp. XZMQ, in subcrust soil, As biotransformation gene aioA was the most abundant, followed by arsM. The dominant phyla of soil biota were Proteobacteria, Cyanobacteria, Actinobacteria, and Bacteroiota, which could play critical roles in shaping aioA and arsM harboring microbe communities in soil. Redundancy analysis (RDA) showed that soil organic carbon (OC), pH, and chlorophyll a (Chl a) were the most important environmental factors in altering soil bacterial communities. Correlation analysis showed the Leptolyngbya had a positive correlation with Chl a, effective nitrogen (N(a)), electrical conductivity (EC), OC, pH in the soil, respectively, while it had a significant negative correlation with As(a), As(III) and As(T). These results emphasized on the significance of cyanobacteria in the behavior of As in mine soils and offered a promising strategy for bioremediation of As-contaminated soil in the mining area.